Nothing to do, so took sometime to do a quick noise profile for my Canon 450D DSLR. Posted the result here for those who might be interested.
Anyway, I took all the shots at night in my room, so the temperature might be slightly higher than outdoor. Also, the DSLR is fully rested before took all the test shots. Below are the procedures:
1. Took 60 shots of a specific ISO with 60s exposure time at 10s interval to simulate normal shooting of light frames.
2. Took another 25 shots of the same specific ISO with 60s exposure time at 10s interval to simulate shooting of dark frames.
3. Median the 25 dark frames together to get the master dark frame of that ISO.
4. Measure the thermal noise on every of the 60 light frames before and after master dark frame subtraction.
5. Median stack the 60 light frames together to simulate normal stacking without dark frame subtraction. Measure the result.
6. Median stack the 60 light frames after master dark frame subtraction together to simulate normal stacking with dark frame subtraction. Measure the result.
Below are the graph of the result:
1. Noise profile before noise reduction (60 x 60s exposure)

2. Noise profile after noise reduction (60 x 60s exposure)

3. Noise profile after stacking (60 x 60s exposure)

Have a nice day.